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Laboratory Diagnostics

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Discover the importance of laboratory testing in veterinary healthcare. Learn about sample collection, handling, basic laboratory procedures, diagnostic techniques, and the role of clinical testing in identifying animal diseases. This unit builds foundational knowledge for accurate diagnosis and effective animal health management.

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What's Inside This Book

Table of Contents

2 chapters, 113 topics

    1. 1.1Laboratory PrecautionsPage 1
    2. 1.2Laboratory Equipment – Their UsesPage 3
    3. 1.3Important Chemicals Used in Laboratories and Veterinary ClinicsPage 47
    4. 1.4FumigationPage 49
    5. 1.5Surgical Instrument SterilizationPage 50
    6. 1.6Ethylene Oxide GasPage 51
    7. 1.7Classification of AntisepticsPage 52
    8. 1.8Oxidizing and Reducing AgentsPage 53
    9. 1.9Alcohols and PhenolsPage 54
    10. 1.10HalogensPage 55
    11. 1.11Antibiotics and Other Antiseptic TypesPage 58
    12. 1.12AcidsPage 59
    13. 1.13Sample Collection and Transport RegulationsPage 64
    14. 1.14Preparation of Standard Solutions Used in the LaboratoryPage 71
    15. 1.15ColorimeterPage 75
    16. 1.16Estimation of Blood GlucosePage 77
    17. 1.17Glucose Quantity CalculationPage 79
    18. 1.18Estimation of Blood CalciumPage 81
    19. 1.19Estimation of Blood PhosphorusPage 85
    20. 1.20Collection, Storage, and Testing Methods of Urine SamplesPage 88
    21. 1.21Identification of Crystals, Blood, and Haemoglobin in UrinePage 92
    22. 1.22Milk ExaminationPage 94
    23. 1.23Collection of Feed Samples and Analysis of NutrientsPage 100
    24. 1.24Bacterial Culture TechniquesPage 104
    25. 1.25Anaerobic Bacteria Culture MethodsPage 107
    26. 1.26Preparation and Identification of Pure CulturePage 108
    27. 1.27Bacterial Biochemical ReactionsPage 114
    28. 1.28Voges–Proskauer Test (VP Test)Page 116
    29. 1.29Indole Production TestPage 117
    30. 1.30Nitrate Reduction TestPage 119
    31. 1.31Methylene Blue Reduction TestPage 120
    32. 1.32Catalase TestPage 121
    33. 1.33Oxidase TestPage 122
    34. 1.34Culture Media for BacteriaPage 122
    35. 1.35Smear Staining MethodsPage 123
    36. 1.36Types of Staining MethodsPage 124
    37. 1.37Acid-Fast Staining Method (Acid-Fast Staining / Ziehl–Neelsen Staining)Page 128
    38. 1.38Special Staining Techniques for BacteriaPage 129
    39. 1.39Capsulated BacteriaPage 131
    40. 1.40Antigen–Antibody ReactionsPage 134
    41. 1.41Types of Antigen–Antibody ReactionsPage 146
    42. 1.42Precipitation ReactionPage 147
    43. 1.43Types of Gel Diffusion TestsPage 149
    44. 1.44Agglutination TestPage 150
    45. 1.45Abortus Bang Ring Test (ABRT)Page 154
    46. 1.46Indirect Haemagglutination TestPage 154
    47. 1.47ComplementPage 156
    48. 1.48Enzyme-Linked Immunosorbent Assay (ELISA)Page 158
    49. 1.49Ascoli's Test for AnthraxPage 161
    50. 1.50Brucellosis Diagnostic TestsPage 161
    51. 1.51Tuberculin TestPage 164
    52. 1.52Johne's TestPage 165
    53. 1.53Mallein TestPage 165
    54. 1.54Samples Required for the Diagnosis of Various Bacterial DiseasesPage 166
    55. 1.55Samples to Be Collected for the Diagnosis of Viral DiseasesPage 170

    1. 2.1Post-Mortem Examination / NecropsyPage 176
    2. 2.2Procedure to Initiate Autopsy in ChickensPage 184
    3. 2.3Tissue Preparation and Section CuttingPage 186
    4. 2.4Hematoxylin & Eosin (H&E) StainingPage 188
    5. 2.5Special Staining TechniquesPage 189
    6. 2.6MicrotomePage 189
    7. 2.7Museum Specimen PreservationPage 191
    8. 2.8Laboratory Diagnosis MethodsPage 193
    9. 2.9Practical SyllabusPage 194
    10. 2.10Mounting and Securing MethodPage 196
    11. 2.11Major Parasites by Organ SystemsPage 197
    12. 2.12Rules to Be Followed in AutopsyPage 201
    13. 2.13Histopathological ExaminationPage 204
    14. 2.14Tissue Processing Before Section CuttingPage 205
    15. 2.15Haematoxylin–Eosin (H&E) Staining ProcedurePage 208
    16. 2.16Blood Smear Staining MethodsPage 210
    17. 2.17Leishman's Staining ProcedurePage 210
    18. 2.18Giemsa StainPage 211
    19. 2.19Requirement of Protozoal CulturePage 214
    20. 2.20Flotation-Based Egg Recovery TechniquesPage 219
    21. 2.21Permanent Staining Methods for Faecal SmearsPage 224
    22. 2.22Collection of Parasitological SpecimensPage 225
    23. 2.23Surgical Terminology (Important Surgical Terminology)Page 229
    24. 2.24Surgery – IntroductionPage 230
    25. 2.25Halsted's Principles of SurgeryPage 235
    26. 2.26Surgical InstrumentsPage 239
    27. 2.27Suture MaterialsPage 250
    28. 2.28Surgical Knots and HitchesPage 261
    29. 2.29Methods of Animal Restraint and CastingPage 264
    30. 2.30CastingPage 267
    31. 2.31Indian / Farrier Casting MethodPage 270
    32. 2.32Reuff / American Casting MethodPage 271
    33. 2.33Buffalo Casting MethodPage 272
    34. 2.34Drugs That Induce Anaesthesia (Anaesthetic Drugs)Page 274
    35. 2.35Pre-Anaesthetic AssessmentPage 275
    36. 2.36Surgical PackPage 280
    37. 2.37Main Surgical Instruments in a Surgical Pack (Surgical Instruments in a Surgical Pack)Page 281
    38. 2.38Methods of Sterilization of Surgical InstrumentsPage 283
    39. 2.39Precautions While Using an AutoclavePage 289
    40. 2.40Classification of Antiseptics and DisinfectantsPage 294
    41. 2.41Pre-Operative and Post-Operative PrinciplesPage 296
    42. 2.42InflammationPage 303
    43. 2.43WoundsPage 306
    44. 2.44AbscessPage 314
    45. 2.45Cyst (Cyst / Fluid Cyst)Page 317
    46. 2.46Tumours / Cancer LumpsPage 320
    47. 2.47UlcersPage 326
    48. 2.48NecrosisPage 329
    49. 2.49GangrenePage 331
    50. 2.50BurnsPage 333
    51. 2.51HerniaPage 336
    52. 2.52Yoke GallPage 341
    53. 2.53FracturesPage 344
    54. 2.54Stages of Bone HealingPage 347
    55. 2.55Dressing MaterialsPage 349
    56. 2.56Bandage TechniquesPage 351
    57. 2.57Method of Dressing Different Types of WoundsPage 354
    58. 2.58Seed Pressing (Castration)Page 356

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